1H J-Resolved Spectroscopy to Evaluate 1H-1H and 1H-19F Coupling Constants

2D ¹H J-RESolved spectroscopy (JRES) is able to separate the ¹H chemical shift and J coupling interactions in the F2 and F1 domains of the 2D data, respectively. The F2 projection represents the pure-shift ¹H decoupled ¹H NMR spectrum while the individual F1 slices at each chemical shift reveal the ¹H - ¹H J coupling for each resonance.  When this technique is applied to a spin system with both homonuclear ¹H-¹H coupling and heteronuclear coupling, it has the ability to provide both the homonuclear and heteronuclear coupling constants.  This is demonstrated in the figure below for 2,3-difluoro pyridine which has both ¹H-¹H and ¹H-¹⁹F coupling.

The top trace in the figure is the ¹H NMR spectrum showing the complex resonances due to both the homonuclear and heteronuclear coupling.  The 2D JRES spectrum is highlighted in grey.  The ¹H-¹H coupling is shown in the F1 slices which were summed to produce the blue, red and green vertical traces in the figure for ¹H resonances A, C and B, respectively.  These traces are identical to the resonances in the separately collected ¹H spectrum with ¹⁹F decoupling shown in the bottom trace of the figure.  The F2 projection of the JRES spectrum is shown in the trace directly on top of the 2D spectrum, colour coded in yellow.  The F2 projection represents the ¹H decoupled ¹H spectrum showing only the ¹H- ¹⁹F coupling.  It can be compared to the separately collected PSYCHE pure-shift ¹H spectrum, colour coded in orange which is very nearly identical.  Clearly this very simple, often overlooked, technique can provide a great deal of both homonuclear and heteronuclear coupling information.