University of Ottawa NMR Facility Web Site

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Wednesday, March 4, 2015

BLOG Index

University of Ottawa NMR Facility BLOG INDEX
March 2015

               H20 vs D2O
1D selective HOESY
               for exchanging roramers
1D TOCSY measurements
               as a function of mixing time
               for mixture analysis
1H NMR with X nucleus decoupling
2D correlation spectroscopy (COSY)
               11B COSY
               COSY 90 vs COSY 45
               double quantum filtered
               ECOSY for measurement of coupling constants
               magnitude vs phase sensitive
               vs TOCSY
               and NOE (1)
               and NOE (2)
               better data – more scans or more slices?
               data apodization
               phasing (video tutorial)
2D NOESY measurements
               19F NOESY
               and exchange (1)
               and exchange (2)
               choice of mixing time
               effect of viscosity
               phasing (video tutorial)
               small vs large molecules
               HSQC - TOCSY
               TOCSY vs COSY
2H NMR of liquids
               chemical shift referencing in
               on Bruker AVANCE spectrometers
               backward linear prediction to correct for
               pulse sequence to minimize
ASCII file generation
               in TOPSPIN (1)
               in TOPSPIN (2)
Background signals
               from dirty NMR probe
Background suppression
               11B in liquids
               11B in solids
Baseline correction
               in 2D NMR spectra
               in solids MAS spectra of quadrupolar nuclides
Benchtop NMR
Chemical exchange agents for spectral simplification
               D2O shake
               trifluorocaetic acid
Chemical shift referencing
               1H in aqueous solution
               in 2H NMR
Chemical shift tensors
               and MAS sideband manifolds (1)
               and MAS sideband manifolds (2)
               from static CP spectra
               measurement from static solids spectra
Chemical shifts
               concentration dependent
               temperature dependent
Concentration gradients
               effect on spectral quality (1)
               effect on spectral quality (2)
               13C – 14N
               13C – 19F
               13C – 2H (1)
               13C - 2H (2)
               13C – 2H (3)
               13C - 59Co
               signs of coupling constants vis ECOSY
               CPMAS of household dust
               effect of contact time
               effect of MAS spinning speed
               importance of Hartman-Hahn match
               in relation to MAS and high power decoupling
               measurement of 13C 90 degree pulses with
               measurement of relaxation times with
               optimizing 1H decoupling in
               ramped contact pulses
               sensitivity improvement from
               to distinguish solid polymorphs
               vs Bloch decay
               with FSLG HETCOR
               as an assignment tool
               effect of spinning speed
               with long dephasing delays (1)
               with long dephasing delays (2)
Decoupler pulse calibration
               in liquids
               in solids
               1H and 31P decoupling
               1H decoupling and 13C signal-to-noise ratio
               1H decoupling and 31P signal-to-noise ratio
               11B decoupling
               13C decoupling
               19F decoupling
               2H decoupling
               27Al decoupling
               31P decoupling
               high power 1H decoupling in solids
               homonuclear decoupling
               modes of heteronuclear decoupling in liquids
               and quaternary alkyne carbon sites
               APT vs DEPT 135
               effect of 1H tuning on
               missing signals in
               of “acetone-d6”
               of “perdeuterated “ solvents
               vs DEPTQ
               with 29Si
               vs DEPT
Diffusion and DOSY
               diffusion in CPMG measurements
Dynamic processes studied by 1D NMR
               exchange studied by saturation transfer
               exchanging rotamers
               the NMR time scale
               Fourier transform of
               gradient spin echoes and selective excitation
               simple spin echo
               to remove 11B background in solids
               tutorial video
               tutorial video
Floor vibrations
               effect on NMR spectra
Food and drink
               candy cane
               fruit cake
               rum and eggnog
               shortbread cookies
Free induction decay
               effects of truncation
HMBC experiments
               HMQC responses in HMBC spectra
               measuring  19F – 13C coupling in 1H - 13C HMBC
               missing signals in              
               Nyquist fold-backs in
HMQC, HSQC and edited HSQC experiments
               1H – 11B
               19F – 13C
               31P -109Ag
               31P – 13C
               doubled signals / poor decoupling
               HMQC vs HSQC
               HSQC - TOCSY
               HSQC vs edited HSQC
               isotope effects in 19F – 13C HMQC
               removing t1 noise in
               Bloch-Siegert shifts
               broadband 1H decoupled 1H spectra
iPad / iPhone apps
               Bruker Almanac
Isotope effects
               complexed solvents
               in 19F – 13C HMQC
               methylene chloride
               “perdeuterated” solvents
               triphenyl phosphate
Linear prediction
               backward LP to correct for receiver overload
               forward LP for 2D data
               consequence of locking on the wrong solvent
               how high should the lock signal be?
               paramagnetic samples
               spectra acquired with a sweeping field
               spectral distortion from weak lock signal
               1H MAS
               and available rf field
               effect on Hartman-Hahn match
               field dependence of chemical shift resolution
               how fast to spin
               how much sample
               increasing signal-to-noise ration in
               setting the magic angle (1)
               setting the magic angle (2)
               solution vs MAS spectra
               rotor crashes
               accessing a used magnet
               measurement of field drift
Magnet cryogen fills and spectral quality
               helium fills
               helium one-way valve oscillation
               nitrogen fills
               nitrogen pressure in the magnet
Magnetic resonance imaging (MRI)
               gradient calibration / 1D MRI
               MRI photocopier
               Nyquist fold-backs in MRI images
               slice selection
               why MRI scanners are loud
NMR of more than one isotope
               23Na and 51V
NOE’s and decoupling
               negative NOE’s and decoupling
               positive NOE’s and decoupling
               and digital filtering
               and mode of data acquisition
               in HMBC data
               in MRI
               13C spectra of paramagnetic compounds
               determination of paramagnetic susceptibility
               paramagnetic shifts
               the effect of paramagnetic oxygen
               first order phase errors
               phasing a 1D spectrum (video tutorial)
               phasing a 2D spectrum (video tutorial)
               broadband vs inverse broadband
               coil geometry
               why so expensive
               effect of 1H tuning on 13C spectra
               effect of cable length
               effect of sample spinning
               effect on 90 degree pulse
               probe electronics
               problems with salty samples
               effect of probe tuning
               fast determination
               for shaped pulses              
               for spin I = n/2 quadrupolar nuclei in solids
               expressed in dB Bruker vs Varian
               expressed in Hz  
Pulsed field gradients
               dephasing ability
               gradient spin echoes
               recovery times
               distortions in QCPMG spectra
               for solid state 2H NMR
Quadrature spikes
               how to remove
               finding lost 13C signals
Receiver gain
               and signal-to-noise ratio
               distortion from mis-setting
               effect of paramagnetic oxygen
               faster relaxation time measurements in solids
               T1 anisotropy in solids
               T1 measurement
               T2 CPMG filter to enhance sharp lines
               T2 measurements and diffusion
               T2 vs T2*
               by using benzene as a solvent
Sample limitation
               making the best of
               and signal-to-noise ratio
               broadband 180 degree pulses
               excitation profiles (1)
               excitation profiles (2)
               for selective excitation
               effect of concentration gradients
               effect of sample depth
               effect of sample mixing
               effect of sample volume
               line shapes resulting from bad shimming
               without a lock signal
               1H spin pairs
Solid state 2H NMR spectroscopy
               echoes and Fourier transforms
               increasing signal-to-noise ratio
               measuring spectral parameters
               T1 anisotropy
               to determine molecular motions
               90 degree pulse calibration
               baseline correction
               field dependence
               Fourier transform of a single rotational echo
               satellite transitions
Solvent effects
               improving resolution
Solvent suppression
               absolute water suppression
               double presaturation
               watergate vs presaturation
Spin simulations
               isopropyl groups
               quadrupolar line shapes in solids (QUEST)
               virtual coupling
t1 noise removal in 2D spectra
               heteronuclear (tutorial video)        
               homonuclear (tutorial video)
Triple resonance experiments
               13C [2H][1H]
               13C [31P][1H]
               31P -13C HMQC with 1H decoupling
Variable temperature measurements
               temperature calibration (1)
               temperature calibration (2)
               temperature dependent chemical shifts
               temperature gradients
               variable temperature to improve resolution
Video tutorials
               1D spectrum phasing
               2D spectrum phasing
               exponential line broadening
               EZ NMR
               removing t1 noise from homonuclear 2D spectra
               and throwing away noise


Ge "David" Feng said...

This is great! Thanks for delivering all the helpful info over the years!


university said...

A debt of gratitude is in order for conveying all the supportive data throughout the years!

membrane protein  said...

Thanks for sharing us these information!

Anonymous said...

Dear Glenn

I have an issue with low content impurities in the presence of a main ingredient, so I would know which is the dynamic range for my experiments on a Bruker Avance 400 MHz equipped with 16 bit ADC.
I mean which is the minimum signal area that is detectable in presence of a major peak, number of scans are not a problem but I'm afraid that the higher signal could flatten the smaller one.

Thank you very much


Glenn Facey said...

The dynamic range is very instrument and hardware dependent. It also depends on the way in which digital filtering and oversampling are used as well as the receiver gain used. If you need an accurate determination of the dynamic range, I recommend that you contact Bruker with the specific details of your instrument and data collection methods.


Unknown said...

Hello Prof. Glenn Facey,
I am working with HR-MAS NMR, 400 MHz (BRUKER), I have problem to shim the magnet, shimming is not working completely, I used the preliminary shimming results too,even the LOCK SIGNAL doesn't change it's position neither up nor down. Actually I changed the rotor, sample to sample, solvent, probe and set again, restart the BOSS, restart the whole system but in vain.
Spinning rate, 5kHz
Temperature, 23 C.

You kindly have any recommendation(s) ? please.

Glenn Facey said...

Ali Sher,
Does adjusting the shims have any effect at all? If the shims are having absolutely no effect, I would check the connections at the shim power supply and at the base of the shim set in the magnet. Are the connections good? Perhaps you have a problem with the shim power supply.


Unknown said...

Thanksgivings dear Prof. Glenn Facey, for your great & in no time response, actually I checked all the connections too, which sound well connected and adjusted certainly there is no effect towards shimming. Might be it'd have certain potent problem, we are fighting to identify and solve.

Gratefulness once even more...
Best wishes.